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Currently innovations are necessary elements of economic growth and competitiveness of companies as well as they are necessary elements of global sustainable development. Even though it is possible to find a wide rouge of definitions of innovation but every one of them is based on different requirements. The aim of submitted article is to compare different approaches to definition of the content of innovation. The authors compare various definitions here and by use of analysis and synthesis, they determine important common elements which are possible to be found in various forms in a number of existing definitions. Then, these elements are compiled into own definition of the content of the term innovation.

Wind erosion, common problem of light-textured soils, was determined on heavy clay soils in the foothills of Bílé Karpaty Mountains, Czech Republic. Soil erodibility by wind was determined from the Map of potential erodibility of soil by wind and from the calculation of potential and real soil loss by wind. All the determinations show underestimation of soil erodibility by wind on heavy clay soils, because methods that are used for this are based above all on the assessment of clay particles content and the presumption the more clay particles soil contains, the less vulnerable to wind erosion is. The potential erodibility of soil by wind is 0,09 t.ha^-1 per year. The determined value does not exceed the tolerable soil loss limit 10 t.ha^-1 per year for deep soils. The real average erodibility of soil by wind has the highest value 1,47 g.m^-2 on November 30^th, 2008. Other soil losses that do not exceed the tolerable soil loss limit 1,4 g.m^-2, were determined on March 18^th and 28^th, 2008. Big difficulties come with the assessment of the erodibility of heavy clay soils in the areas, where soil erosion verifiably exists, but it is not assessable by objective calculating methods. Evident necessity of new knowledge concerning the determination of wind erosion intensity follows from the results.

Risk is the one of basic and inevitable characteristics of every human activity. In connection with business activities, it is the factor that is not possible to entirely eliminate. The fact that it is possible only to minimize a risk, respectively to minimize its negative consequences at certain conditions fulfilment is one o the risk specifications.
The problem of risk classification is seriously wide. In scientific literature, various categorisations of risk are described and discussed while the categorisation is usually based on the purpose of presented classification.
Risk categorisation represents the file of a risk into the one of existing categories constructed on the basis of parameters of classified risks. In the work, the authors summarize the theoretical backgrounds of risk categorisation, and it is especially in consequences of risk connected with business activities.
The objective of the article is to provide the synthesis of theoretical categorisations of risk that would be contributing to the discipline of risk management as to the scientific discipline.

The aim of this study was to evaluate the possible effects of maize replacement in feeding rations on the amino acid content in cows' milk. Cows were fed total mix ration based on the maize, clover silage and hay. There was a difference in the concentrate of the feeding ration. The first group (fed maize) was the control group, another two groups were experimental, one fed wheat and second fed triticale. During six weeks, totally 26 milk samples were taken from dairy cows of Czech Pied breed. Feed groups were preferably balanced in terms of milk yield, stage and number of lactations. The samples of feedstuffs as well as milk were modified for the analysis using acidic and oxidative hydrolyses. The analysis of amino acids content and composition of the sample hydrolysates was performed chromatographically by an AAA 400 analyzer, using Na-citrate buffers and ninhydrin detection. Total nitrogen content was determined according to Kjehldahl and the crude protein of the samples was determined by conversion from the nitrogen content multiplied by appropriate factor. The high content of crude protein in wheat did not influenced composition of milk from dairy cows fed this type of feedstuff. With respect to resulting amino acid content and composition of milk samples, none of the tested grains can be recommended as a full-value maize replacement. Each feedstuff is an abundant source of several and typical amino acids in milk. However, triticale (cultivar Kitaro) seems to be acceptable replacement of maize owing to better crude protein efficiency, composition and health indicators of milk quality.

Casein measurement is important for cheesemaking and control of dairy cow nutrition. Reference Kjeldahl method is not suitable for routine purposes. Infra-red spectroscopy MIR and MIR-FT use can be a solutin. However, their casein specifity is relatively limited. Aim of the work was to assess the quality of performed calibrations for validation of calibration parameters. A retrospective analysis of MIR and MIR-FT calibrations was performed for estimation of limits their suitable parameters. Mean casein values of reference sample sets varied from 2.49 to 2.7% (2.61 ± 0.155). Mean variation range was 0.561 ± 0.164%. The mean correlation coefficient of calibration (KKK) was 0.974 ± 0.018 (P < 0.001). The mean standard deviation of mean for individual differences (SDID) was 0.03 ± 0.011% (from 0.01 to 0.08). MIR-FT results were slightly better both for calibration and for proficiency testing. The high and low KKKs were higher in the case of accepted calibration as in proficiency testing for MIR-FT and MIR (0.986 > 0.964 and 0.970 > 0.948; 0.982 > 0.947 and 0.947 > 0.911; P < 0.001). The casein number varied from 79.4 to 80.56% in bulk milk samples in three years, its variability was low from 1.4 to 1.5% relatively, which shows on relatively reliable casein analyses by methods MIR and MIR-FT. It does not need to agree fuly for individual milk samples. It is linked more to MIR than MIR-FT. Limits for acceptable calibration parameters were derived: > 0.945 for KKK; 0.048 for SDID and 0.029% for mean difference as maximum.

Heavy clay soils that are normally resistant to wind erosion, from study site Ostrožská Nová Ves situated in the foothills of the Bílé Karpaty Mountains, Czech Republic, were a subject of laboratory analyses. The analyses should found out the influence of overwinter processes on disruption of soil aggregates and thus reason of vulnerability to soil loss by wind. Two overwinter processes were observed - freezing and thawing, and freeze-drying of the soil. Both processes have indicated the increasing of erodible fraction in dependence of water content of analysed soils. Exposed frozen clay soils that freeze-dries during the winter in the foothills of Bílé Karpaty, leaves soils highly erodible in late winter and early spring.

The new method of the evaluation of the mechanical behaviour of fats has been designed. This method is based on the evaluation of the fat response to dynamic loading. Dynamic loading has been realized by fall of bar impact. Response function has been represented by the time history of the fat surface displacement. Response of the tested table fats have been evaluated both in the time and frequency domain. Two factors affected rheology of the examined fat products: temperature of the measurement and product origin. The influence of fat content can be described namely in the frequency domain. The results obtained between 14 and 20 °C showed significant differences in the fat rigidity.

Milk freezing point (MFP) is important quality indicator. Aim was to analyse the relationships of MFP to selected udder health milk indicators (MIs) by comparison between cows (reference), goats and sheep. Bulk milk samples came from 3 herds of Czech Fleckvieh (B, n 93) and 1 goat herd and sheep flock (White short-haired, W, n 60; Tsigai, C, n 60). Animal nutrition was performed under the typical country conditions. MIs which were investigated: DM, dry matter; SNF, solid non fat; L, lactose (all in %); SCC, somatic cell count (10³ ml^-1); EC, electrical conductivity (mS cm^-1); MFP (°C); Na and K (in mg kg^-1). W MFP was -0.5544 ± 0.0293, B -0.5221 ± 0.0043 and C -0.6048 ± 0.0691 °C. The B MFP was related to L (-0.36; P < 0.01), W was not related to L (-0.07; P > 0.05) and C was related to L (0.40; P < 0.01). These facts could be explainable by worse SCC geometric averages for used W (3,646 10³ ml^-1) and C (560 10³ ml^-1) milk as compared to B (159 10³ ml^-1). Only 0.5 and 10.5% of variations in MFP were explainable by variations in DM and SNF in B, 32.7 and 12.8% in W but already 49.4 and 45.0% in C. Higher C values were caused by high MFP variability, 11.8% (C) versus 0.8% (B). There is possible to derive the more reliable MFP qualitative limits for more efficient monitoring rules of milk quality problems in B, W and C.

Cambisols cover an estimated 45% of agricultural soils in the Czech Republic. We aimed our work at stabile forms of organic carbon and humic substances quality in Cambisols under different types of soil management (grassland and arable soil). Object of our study were the following subtypes of Cambisols: Eutric Cambisol (locality Vatín - arable soil), Eutric Cambisol (locality Vatín - grassland), Haplic Cambisol (locality Náměšť n/Oslavou - arable soil), Leptic Cambisol (locality Ocmanice - grassland), Haplic Cambisol (locality Nové Město na Moravě - arable soil), Haplic Cambisol (locality Přemyslov - Tři Kameny - grassland), Arenic Cambisol (locality Pocoucov - arable soil), Dystric Cambisol (locality Sněžné - arable soil), Dystric Cambisol (locality Velká Skrovnice - arable soil), Dystric Cambisol (locality Vojnův Městec - arable soil). Non-destructive spectroscopic methods such as UV-VIS spectroscopy, synchronous fluorescence spectroscopy (SFS) and ¹³C NMR spectroscopy for humic substances (HS) quality assessment were used. Total organic carbon (TOC) content was determined by oxidimetric titration. Fractionation of HS was made by short fractionation method. Isolation of pure humic acids (HA) preparation was made according to the standard IHSS method.
Results showed that TOC and humus content varied from 2.70 % (grassland) to 1.3 % (arable soil). Average HS sum was 8.4 mg/kg in grassland and 6.4 mg/kg in arable soil. Average HA sum was 3.6 mg/kg in grassland and 3 mg/kg in arable soil. Fulvic acids (FA) content was 4.7 mg/kg in grassland and 3.7 mg/kg in arable soil. HS quality was low and very similar for all studied samples. HA/FA ratio low (< 1). HS absorbance in UV-VIS spectral range was low and similar in all studied samples. Higher absorption in this spectral range was closely connected with higher HS content. Also in 2D-synchronous fluorescence scan spectra similar shape of spectral lines was detected. Relative fluorescence intensity strongly depended on HA and FA content. Five main fluorescence peaks for HA and FA presence at λ_ex./ λ_em.: 468/488, 482/502, 450/470, 376/396, 340/360 at constant difference of Δλ=20 nm were measured. ¹³C NMR spectra of HA isolated from Eutric Cambisol showed low aromaticity degree and high content of aliphatic moieties. HA were young (= less aromatic compounds in HA molecule) and no differences in HA structure were detected to compare arable soil and grassland.

Effect of addition 6% of linseed oil (designated L06), 6% and 10% of fish oil (R06 and R10) to feed on the fatty acid spectrum of common carp (Cyprinus carpio L.) was investigated. The basic feedmixture which was used as a control variant (K - 34% protein; 9% fat) and the three with oil addition (L06, R06 and R10) were fed to carp fingerling (43.25 g average weight) for 60 days - from 23^rd April to 20^th June. Before that the fish were fed for 2 month by whey grain and commercial feed for carp fingerling in pond fish-culture (KP feed mixture - 33% protein; 5% fat) at daily feeding rate 1.5% of actually fish mass. This procedure was intended to create feeding conditions closest to those witnessed in market fish farmed in ponds during the vegetation season nevertheless the spectrum of fatty acids present in the fish muscle at the experiment's beginning did not fully correspond to what was observed in carps living in ponds and fed by cereals.
An addition of 6% of linseed oil to the feed lowers the content of the oleic acid and MUFA and, at the same time, it boosts the contents of the α-linoleic acid, n-3 PUFA and the general PUFA in the meat of carp fed on mixtures thus enriched. Additions of 6% and 10% of fish oil to the feed for common carp increases the content of the eicosapentaenoic acid. The 10% addition proved beneficial for also the ratio of n-3/n-6 PUFA. The high content of the docosapentaenoic acid and the general PUFA in the meat of fish as early as the beginning of the experiment resulted in a smaller number of significant changes in the spectrum of fatty acids (particularly the docosahexaenoic acid, PUFA and n-3/n-6 PUFA) found in the fish meat of the L06, R06 and R10 experimental variants.

Evaluation of milk yield, composition and quality of organic milk of crossbreeds of Lacaune (L), East Friesian (EF) and Improved Wallachian (IW), (n = 10, L 50 EF 43.75 IW 6.25) during lactation was carried out on organic farm in Valašská Bystřice in 2007. All sheep were on the third lactation and during study they were reared on permanent pasture. The stage of lactation (SL) had a highly significant effect on milk yield (MY) and contents of total solids (TS), fat (F), protein (P), casein (C), lactose (L) and urea (U). The SL had also a highly significant effect on pH and titrable acidity (TA), whereas on rennet clotting time (RCT) the SL had a significant effect. On the other hand the SL had not a significant effect on somatic cell counts (SCC) and rennet curd quality (RCQ). The highest contents of TS, F and C were found at the end of the lactation, whereas their lowest contents were found on the 120^th day. Between the second and the last sampling the lactose content decreased. The content of U and RCT were the most variable indicators whithin the frame of our study. On the other hand SCCs were, during the whole lactation, very well-balanced and relatively low. Between pH and SCC was found positive correlation. TA gradually increased and RCT gradually prolonged with advanced lactation.

In small-plot experiments established in 2001-2004 we studied the effect of the chemical composition of the plant dry matter of spring barley, varieties Kompakt and Jersey, on grain yields. A relatively strong correlation was confirmed between the chemical composition of the plant dry matter and yields. The correlation was most intensive in the case of nitrogen (r = 0.536), phosphorus (r = 0.503), magnesium (r = 0.464) and sulphur (r = 0.431) at the beginning of shooting (DC 30); in the case of potassium (r = 0.557) at the beginning of tillering (DC 23) and calcium (r = 0.530) during ear formation (DC 55). A relatively strong correlation remained from the beginning of tillering to the beginning of ear formation and later decreased. The weather conditions of the year and variety significantly affected grain yields and also the previous crop was important. Grain yields of the variety Kompakt were statistically significantly lower than of the variety Jersey (6.02 t.ha^-1 and 6.45 t.ha^-1, respectively). The yields of barley grown after sugar-beet were the highest (6.30-6.79 t.ha^-1); the grain yields of barley after maize decreased by 9.1-9.7 %. Higher grain yields of the Jersey variety resulted in levels of N-substances (11.35 %) lower than in the Kompakt variety (11.35 % and 11.60 %, resp.). No correlation was discovered between the nitrogen level in the plant dry matter during vegetation and the content of N-substances in barley grain.

Nowadays wood modification is very-discussed and its importance is growing up. Wood plasticization alias a temporary change of wood properties makes subsequent processing possible. Plasticization is very suitable for subsequent mechanical compressing of wood. Instead steaming and boiling there is a microwave heating for wood plasticization. This kind of heating cuts down the time of plasticization from hours to seconds and also reduces energy consumption.
Pressing of wood perpendicular to the gain in radial direction causes change of wood structure, increases wood density and due to this the wood has increased mechanical properties as bending strength and Young's modulus of elasticity. Modificated wood has many positive properties, but also has number of negative properties. The high-temperature treatment eliminates these negative proeprties to the large extend. The high temperature causes changes in chemical structure and reduces number of soprtion places. Due to this the wood has lower equilibrium moisture content. Moisture content greatly affects physical and mechanical properties. The wood that was treated by high temperature (200 °C) has lower moisture content coresponding to the equilibrium moisture content than the wood without high-tepmerature treatment (the wood only microwave plasticizated and mechanicaly compressed). Lower moisture content makes rising bending strength and Young's modulus of elasticity.
The aim of this article is to compare bending strength perpendicular to the gain in radial direction and Young's modulus of elasticity of Spruce wood that was microwave, mechanical and modificated in high-temperature.

This article attempted to define a compromise making it possible the satisfaction of the material needs of the populations living within the National park of Salonga while ensuring the conservation of long-term forest resources. The management of the forests requires deepened knowledge of the resources and the participation of the local communities, which are the better, informed on of the forest resources. The implementing of a policy on sustainable forest management would be possible by a better integration and participation of the local populations. A survey was carried out in four villages of the National park of Salonga. The results of the investigation show clearly a positive attitude of the rural populations with regard to the forest resources. The diversity of the needs for the population corresponds to the choice of the products and services of the forest. Overall, the potential of the park's forests is superior in comparison with the needs of the population. The exploitation of the forest products is vast and is included in the category of a system of an economy of collection.

Changes in amino acid profile of alfalfa silage preserved with chemical or biological additives were studied in fresh and wilted silage. The chemical additive was formic acid and the biological additive consisted of Lactobacillus rhamnosus, L. plantarum, L. brevis, L. buchneri and Pediococcus pentosaceus. Second cut alfalfa (Medicago sativa L.) was harvested at the bloom stage, ensiled in mini silos (15 dm³) and fermented at 20-23 °C for 12 weeks. The dry matter of the fresh silage was 228 g.kg^-1 and 281.6 g.kg^-1 for the wilted before ensiling. The amino acid content was estimated by using an automatic amino acid analyzer AAA (INGOS Prague). The results of the experiments indicated that amino acid breakdown was inhibited by increased dry matter and the use of chemical and biological additive. Additionally, the content of amino acids was found to change in relation to the degree of wilting and formic acid treatment yielded the lowest amino acid breakdown. The amino acid breakdown was also reduced by biological preservative especially in the silage with a higher level of dry matter content.

The aim of our study was to evaluate the relationship between reproduction and milk traits in cows of the Czech Fleckvieh. In the period of 90-180 days after calving milk of each dairy cow was analysed for constituent milk components and milk features (40 analyses in total for each sample). The database contained data concerning to the origin and reproduction traits (service period, services per conception and interval between calving and first insemination) for every single cow.
When the calculated correlations were evaluated the milk yield reached significant (* p < 0.05) to very significant (**p < 0.01) negative correlation to content of some milk components: fat (-0.253**); rough protein (-0.256**); casein (-0.197**); pure protein (-0.247**); and also to content of some macroelements as: phosphorus (-0.245**); sodium (-0.261**); magnesium (-0.151*).
Relation of milk yield (in kg) and reproduction traits (number of inseminations and length of service period) shows non-significant positive correlations. With increasing of milk yield, there are a higher number of services per conception and longer service period. It confirms a general tendency of deterioration of reproduction with an increasing milk yield.
We have found out that when the concentration of urea, acetone, number of somatic cells and percentage of fat in milk of Czech Fleckvieh cows was increased, reproduction traits worsened (number of inseminations, length of service period). The differences were not statistically significant. This negative relation was not proven to an interval (number of days between calving and the first insemination), where a company management may have a significant influence.

The enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD) has a very important role in the biosynthetic pathway of vitamin E. Its activity influences the final level of tocols in plant tissues. Seven barley cultivars with different vitamin E level were grown under control conditions and activity of HPPD gene was measured four, eight and twelve days after pollination of ear tissues. It was found that activity of HPPD gene corresponded with vitamin E content detected in grains (r = 0.77*). The relationship between the gene activity for HPPD eight and twelve days after pollination and vitamin E content was also confirmed for analyzed cultivars grown in the field conditions (r = 0.85*).

The aim of this study was isolation, identification and characterization of bacteria of the genus Enterococcus from Duch-type semi-hard cheese during manufacture and ripening. Cheese samples from two different producers (I and II) were used at the production day and after 30, 90 and 176 days of ripening.
Altogether 361 suspected enterococci isolates were obtained from cheese samples during 7 month of ripening. Using genus-specific PCR, 285 isolates were identified as the members of the genus Enterococcus. The identification of five Enterococcus species was performed by PCR using species-specific primers. Among 165 Enterococcus spp. isolates of producer I, 81 isolates were classified as E. faecium, 39 as E. durans, 21 as E. faecalis, 19 as E. casseliflavus and 3 as E. hirae, and 2 isolates were not classified into species. Enterococci species among isolates of producer II were as follows: 52 isolates of E. faecium, 38 of E. faecalis, 14 of E. durans, 12 of E. casseliflavus, 3 of E. hirae and 1 was not classified into species. E. faecium was found to be the dominating species in all cheese samples. The gene coding for tyrosine decarboxylase was detected in 10 enterococci isolates of producer I and in 5 enterococci isolates of producer II. Production of biogenic amine tyramine was confirmed in all these isolates, which were of E. faecium, E. faecalis and E. durans species. It was confirmed that these species are important for tyramine production. There is the relationship between tyramine production and counts of E. faecium, E. faecalis and E. durans. No tyramine production was observed in isolates of E. casseliflavus or E. hirae species.

We focused on tomatoes for industrial processing due to its economical importance for its lycopene content. The objective of our research is to find the variation of lycopene content in tomato fruits depending upon the length of after harvest storage and thermic treatment, which is inevitable when being industrialy processed. From the point of view of nutritional qualities the most relevant contentual substance of tomatos are carotenoids, included lycopene.
At average for tree following experimental years we learnt significant differences regarding the content of lycopene and the length of storage of tomato fruits. Immediately after the harvest and proccesing tomato fruits contained, at average for tree years, 103.24 mg of lycopene. After 14 days the content of lycopene declined to 46.76 mg.kg^-1 of fresh mass. After 30 days the average value dropped to 29.26 mg.kg^-1. This fact comfirms that boiling respectively thermic treatment increases the content of lycopene in tomato fruits, particulary in our experiment to the value 83.33 mg.kg^-1. At varieties Ladislav, Peto 86, Prémium, Salus the content of lycopene has even risen in comparison with its content up to 48 hours after the harvest. Tomato (Lycopersicon esculentum L.) belongs to the most signifficant vegetable varieties either for its exploitation in processing industry as well as for its nutritional value with extraordinary beneficial effect for human organism.
Although the content of lycopene is genetically stable attribute, its content in our experiment ranged from 45.39 mg.kg^-1 (Prémium variety) to 77.98 mg.kg^-1 (Zámčan variety), which are significant differences.

Non-significant differences were established when the nutritional and technological beef quality of Czech Fleckvieh and Montbeliard and their reaction to the end of feeding at the different age and different carcass growth intensity were studied.
Bulls were fattened until average weight of 656 kg. Montbeliard beef quality evaluation shown non-significant difference of higher intramuscular fat proportions and energy value compared to Czech Fleckvieh. Technological quality evaluation only indicated the tendency to lighter meat, lower water retention and lower level of hydroxyproline. Slaughter age rising in both breeds (the boundary vas 570 days) resulted in non-significant increasing of intramuscular fat together with significant (P < 0.05) increasing of muscle pigmentation content and non-significant shortening and water retention reduction.
Longer fattened bulls at both breeds shown non-significant m.l.th. area reduction at higher difference at Montbeliard. Net gain level as a carcass growth intensity, showed non-significant intramuscular fat production. Higher level of net daily gain produces, particularly at Montbeliards, lead to increasing of hydroxyproline. Higher growth intensity of Czech Fleckvieh influenced pigmentation content increase. Higher growth intensity in both breeds leads to enlarging the m.l.th.

Defoliation fulfilment in correct terms, range and intensity effects highly on conditions of bunches and quantity of their contentual matters. Due care reduction of foliar surface together with sunshine allows better access of air to the proximity of bunches and their quicker drying up. The development of fungal diseases is not so quick. Notably hold under especially the peril of charging by cluster with Botrytis, whose harvest its possible take away to the later term. Better insolation at the same time abetment by soaking of clusters and degradation of apple acid.
It is possible to make one-shot or gradual defoliation. By smaller growers can the defoliation make by hand, or near bigger with mechanization. In both cases constitute costs on implementation indispensable load sum.

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.
The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production - milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition - milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk - titratable acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.
Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable acidity (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).

The effect of N and P fertilization on the dry matter content, concentration of nutrients (N, P, K, Ca and Mg) and their intake by the plant, production characteristics (achene yields, head diameter, 1000-seed weight, oil content and oil production) and the content of fatty acids (palmitic, stearic, palmitooleic, oleic, linoleic and linolenic) was evaluated in the one-year pot trial with sunflower (Helianthus annuus, L.). Nitrogen application had a positive effect on dry matter production and increased N intake by plants as early as the beginning of vegetation (4^th true leaf). The effect of P on dry matter production was not markedly evident until the later growth stage (10^th true leaf). At the end of the vegetation period the weight of plants of variants fertilised with N only and variants where P was applied evened out. Achene yields, head diameters, 1000-seed weight, oil content and oil production increased statistically significantly after N application. Of all the monitored characteristics the increased level of P in the soil affected only the 1000-seed weight. The application of both nutrients did not significantly change the content of fatty acids.

Fermented pig slurry as a fermentation residue of biogas plants was used for vegetables fertilization as a replacement of industrial mineral fertilizers. Tomatoes were grown in vessels, celery and spinach in the field, radish and lettuce in the greenhouse. Besides dry matter content determination vegetables were evaluated from the food quality point of view. Is consists of microbiological assessment, nitrates and selected elements (Pb, Cd, As, Zn and Hg) content determination.

Drip irrigation constitute one of the general factor by viticulture production. Their wide utilization it is possible expect in warm south exposed regions of southern Moravia. Gist is deficienty of rainfall during the vegetation (about 500 mm), which espressivo yield and qualities performance. Principal aim research design solving on constitution horticultural techniques is verify and valorize influence drip irrigation on growth vine guilty and qualities her performance in climatic and soil conditions of southern Moravia. Subsequently formulate valid findings for users of drip irrigation, which enable their efficient utilization behind contemporary reservation regular, high-quality harvest.

This work deals with use of FT NIR spectroscopy to speedy analysis of maturation of edam cheese. Maturation of cheese was study with the measure of soluble nitrogen, tyrosine and tryptophane. This method can be able to make optimal making raw material to production of processed cheese. Samples of edam cheese (45% fat in dry matter) were analysed with time of ripening 7 to 99 days. Soluble nitrogen, tyrosine and tryptophane were analysed on UV-VisSpectrometer (270 and 290 nm). Sequentially samples were analysed on FT NIR spectrometer of two methods: 1) slice of cheese was analysed help with optical probe, 2) grated cheese was analysed on the integration sphere. The values of correlation coefficient of calibration on the integration sphere were as follows: soluble nitrogen 0.922, tyrosine 0.911 and tryptophane 0.852. The values of correlation coefficient of calibration with optical probe were as follows: soluble nitrogen 0.996, tyrosine 0.958 and tryptophane 0.922.

This work deales with exploitatives parameters monitoring of wood shreder PEZZOLATO 110 Mb by crushing of waste cane of six varieties. The results shows that the wood shreders efficiency, fuel consumption and the wood chips elements size can be influenced by varieties characters of cane. The valued machines efficiency was 230-470 kg.h^-1 by average volume 40.70 % water in wood. The highest values by cane crushing had the variety Saint Laurent (0.47 t.h^-1) and the lowest variety Blauer Portugieser (0.23 t.h^-1). The specific consumption of petrol Natural 95 was 4.52.10^-3-8.12.10^-3 l.kg^-1. The average middle elements lenght was 6.64 mm by crushed varieties.

The aim of the work was to evaluate the effect of addition of humidity absorbent (malt sprouts) and chemical conservation additive on fermentation process quality of brewer grains' silage. Chemical conservation additive was based on formic acid, propionic acid, benzoic acid and ammonium formate content. In a model experiment the fresh brewer grains were used. A dry matter (DM) content of brewer grains was 187.4 g/kg. Six treatments with three repetitions per treatment were prepared. The treatments A₁, A₂ and A₃ were not supplied by humidity absorbent. Treatment A1 was a control treatment without any additive. The treatments A₂ and A₃ were supplied by chemical conservation additive in a dose of 3 L per tonne and 6 L per tonne, respectively. The treatments B₁, B₂ and B₃ were supplied by malt sprouts to reach DM content of conserved matter on level 320-350 g/kg. Moreover the treatments B₂ and B₃ were supplied by chemical additive with its dose 3 and 6 L per tonne. Model silages were evaluated after 8 months of conservation at average laboratory temperature 26-28 °C, from each treatment were the final laboratory samples taken and analyzed. During conservation of treatments B₁, B₂ and B₃ were no drain recognized. From A₁ treatment drained 1300 ml of waste fluid that is 145 L per tonne of conserved matter. That was significant (P < 0.01) the malt sprouts addition support the lactic acid production and eliminate acetic acid production. There was no propionic acid or butyric acid detected in silages with malt sprouts event in these silages were analyzed higher (P < 0.01) concentration of ammoniac. Chemical additive supplementation improved (P < 0.01) the pH value and water leach acidity. The results show the malt sprout addition eliminates waste fluid drain and improves fermentation process. The higher concentration of chemical additive (6 l/t) inhibited the fermentation process in our model experiment.

The influence of the stage of maturity of alfalfa on the chemical composition and in sacco digestibility was studied in a laboratory experiment. Alfalfa plants were analysed in 8 vegetation stages harvested in regular intervals before the beginning of bud setting until the fall of blossoms. The contents of nitrogenous substances, fat, crude fibre, ADF, NDF, Ca, P, Na, K and Mg were determined in the samples, and using the in sacco method also the organic matter digestibility in the rumen. Further we assessed the energy content and calculated the N-free extractives (BNLV) value. In some stages the chemical changes were monitored in the whole plant. It was discovered that during vegetation there was a statistically significant increase in the crude fibre content and in the ADF and NDF fractions and a reduction in nitrogenous substances. A strong negative correlation was detected between the stage of maturity of alfalfa and content of crude protein and net energy for lactation (r = -0.97 and r = -0.92, respectively). In the individual stages of vegetation the differences in the content of nitrogenous substances, fibre and fat were statistically significant (P < 0.05). The statistically highest content of crude protein (28.97%) was detected in the first stage before bud setting and the lowest (11.97%) in the stage after the fall of blossoms. Later vegetation also had a stronger depressive effect on the content of fat and NEL. The organic matter digestibility (P < 0.05) was the lowest in alfalfa in the last stage of sampling (62.6%) when it reached a mere 85.99% of the digestibility at the beginning of bud setting. The highest organic matter digestibility (72.80%) was detected in a sample of young alfalfa prior to bud setting. In the experiment we confirmed a strong and negative correlation (r = -0.97) between the crude fibre content and organic matter digestibility. The correlation was also strong between the stage of maturity of alfalfa and the content of magnesium (Mg).

The paper informs of N, P, K, Ca and Mg-contents in the selected organs (tissues) of 4-year old Norway spruce (Picea abies /L./ Karst.) and 3-year old European beech (Fagus sylvatica L.) transplants after two years of Mg fertilization (100 kg Mg.ha^-1.year^-1) in the form of magnesium sulphate, and N fertilization (100 kg N.ha^-1.year^-1) in the form of ammonium sulphate. Analyses of buds, needles/leaves, bark and wood of above-ground part, fine roots (≤ 1 mm) and small-diameter roots (> 1 mm) showed that the greatest amounts of uptaken nutrients are in both tree species stored in assimilatory organs and in buds. The increased supply of nitrogen showed most in small-diameter roots (spruce), and in root-wood, and wood of stem and branches (beech). The two species responded to the increased supply of magnesium by increasing the bioelement content in root-wood and in fine roots. The increase of Mg-content in leaves occurred only in the second year of the fertilization.