Acta Univ. Agric. Silvic. Mendelianae Brun. 2024, 72(5-6), 177-182 | DOI: 10.11118/actaun.2024.012

Analysis of ˈCandidatus Phytoplasma prunorumˈ titer in the tissues of apricot trees (Prunus armeniaca L.) during the year.

Tomáš Kiss ORCID...1, Gabriela Trempetić1, Peter Morvay1, Tomáš Nečas ORCID...1
Department of Fruit Science, Faculty of Horticulture in Lednice, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

This study focused on monitoring ˈCa. P. prunorumˈ detection rate and titer over the course of the year in one-year-old (January-May) or annual shoots (June-December) of apricot trees. As an experimental material, 3 apricot selections (H990/95, H990/177 and H980/74) each represented by 3 trees of the same age grown in the same orchard were selected and analysed monthly. The real- time PCR assay was used for absolute quantification of ˈCa. P. prunorumˈ. The results showed, that phytoplasma was detectable in high rates (above 90 %) from August until December in annual shoots and from January to March and in May in one-year-old shoots. Lower detection rates were observed in April and in June and July, showing, that these months are not reliable for phytoplasma detection. Phytoplasma titer remained the same from January to May in one-year-old shoots. In annual shoots the lowest titers were observed in June, while in the rest of the year, the titers were significantly higher and not differing from the titers in one-year-old shoots collected from January to May. ˈCa. P. prunorumˈ quantification also showed, that H990/95 selection had significantly lower phytoplasma titer than the H990/177 and H980/74 selections. This was also supported by the detection rates and symptoms, where H990/95 selection was showing lower values of these parameters than the rest of the selections.

Keywords: ESFY, real-time PCR, phytoplasma quantification, stone fruit species

Received: September 23, 2024; Revised: November 26, 2024; Accepted: November 28, 2024; Published: January 1, 2025  Show citation

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Kiss, T., Trempetić, G., Morvay, P., & Nečas, T. (2024). Analysis of ˈCandidatus Phytoplasma prunorumˈ titer in the tissues of apricot trees (Prunus armeniaca L.) during the year. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 72(5-6), 177-182. doi: 10.11118/actaun.2024.012
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    References

    1. CHRISTENSEN, N. M., NICOLAISEN, M., HANSEN, M. et al. 2004. Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging. Molecular Plant-Microbe Interactions. 17(11), 1175-1184. Go to original source...
    2. IRPCM PHYTOPLASMA/SPIROPLASMA WORKING TEAM-PHYTOPLASMA TAXONOMY GROUP. 2004. ˈCandidatus Phytoplasmaˈ, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. International Journal of Systematic and Evolutionary Microbiology. 54(4), 1243-1255. Go to original source...
    3. JARAUSCH, W., LANSAC, M. and DOSBA, F. 1999. Seasonal colonization pattern of European stone fruit yellows phytoplasmas in different Prunus species detected by specific PCR. Journal of Phytopathology. 147(1), 47-54. Go to original source...
    4. KAVIANI, M., GOODWIN, P. H. and HUNTER, D. M. 2024. Pear Rootstock Effects on Seasonal Colonization Patterns of Pear Decline Phytoplasma. Horticulturae. 10(2), 129. Go to original source...
    5. KISS, T. and NEČAS, T. 2022. Quantification of 'Candidatus Phytoplasma prunorum' in apricot trees exhibiting uneven European stone fruit yellows symptoms. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 70(1), 31-36. Go to original source...
    6. KISS, T., ŠAFÁŘOVÁ, D., NAVRÁTIL, M. and NEČAS, T. 2024. Molecular characterization of 'Candidatus Phytoplasma prunorum' in the Czech Republic and susceptibility of apricot rootstocks to the two most abundant haplotypes. Microorganisms. 12(2), 399. Go to original source...
    7. MAIXNER, M., AHRENS, U. and SEEMÜLLER, E. 1995. Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure. European Journal of Plant Pathology. 101(3), 241-250. Go to original source...
    8. MARCONE, C., JARAUSCH, B. and JARAUSCH, W. 2010. Candidatus Phytoplasma prunorum, the causal agent Of European stone fruit yellows: An overview. Journal of Plant Pathology. 92(1), 19-34.
    9. NEČAS, T., KRŠKA, B., MAŠKOVÁ, V. et al. 2008. Influence of the time of sampling on detection of ESFY phytoplasma. Acta Horticulturae. 781, 435-441. Go to original source...
    10. NIKOLIĆ, P., MEHLE, N., GRUDEN, K., RAVNIKAR, M. and DERMASTIA, M. 2010. A panel of real-time PCR assays for specific detection of three phytoplasmas from the apple proliferation group. Molecular and cellular probes. 24(5), 303-309. Go to original source...
    11. SCHAPER, U. and SEEMÜLLER, E. 1984. Effect of the colonization behaviour of the causal agents of apple proliferation and pear decline on their detectability by fluorescence microscopy. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes. 36(2), 21-25.
    12. SEEMÜLLER, E. and SCHNEIDER, B. 2004. ˈCandidatus Phytoplasma maliˈ, ˈCandidatus Phytoplasma pyriˈ and Candidatus Phytoplasma prunorumˈ, the casual agents of apple proliferation, pear decline and European stone fruit yellows, respectively. International Journal of Systematic and Evolutionary Microbiology. 54(4), 1217-1226. Go to original source...

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