Acta Univ. Agric. Silvic. Mendelianae Brun. 2006, 54(5), 149-154 | DOI: 10.11118/actaun200654050149
Molekulární markery genetické variability u odrůd tritikale registrovaných v České republice
- Ústav biologie rostlin, Mendelova zemědělská a lesnická univerzita v Brně, Zemědělská 1, 613 00 Brno, Česká republika
Genetická variabilita byla detekována metodami molekulární genetiky (RAPD a SSR) u 15 odrůd tritikale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR), které jsou registrovány v České republice. Pro detekci polymorfizmu DNA bylo využito 80 RAPD primerů. Nižší reprodukovatelnost RAPD analýz byla kompenzována opakováním analýz (3-4×). Na základě statistického vyhodnocení byl sestaven dendrogram, kde je možno vysoce průkazně odlišit odrůdy Kolor, Modus a Tornado. Zbývajících 12 analyzovaných odrůd tvoří čtyři klastery. Byl optimalizován protokol detekce variability DNA pomocí mikrosatelitních markerů u tritikale. Pro analýzy byly využity dva SSR markery (1A chromozom [Xpsp2999] a 1B chromozom [Xpsp3000]), které byly popsány u pšenice obecné (T. aestivum L.). Na základě použitých dvou SSR markerů se podařilo odlišit odrůdu Triamant od klasterů ostatních analyzovaných odrůd.
tritikale, XTriticosecale Wittmack., DNA markery, RAPD, SSR
Molecular markers of genetic variability in triticale varieties registered in the Czech Republic
Genetic variability was detected in 15 varieties of triticale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR) registered in the Czech Republic by means of polymorphism of DNA using the RAPD method and the SSR method. For detection we used 80 RAPD primers. The lower reproducibility of the RAPD markers was resolved by means of repeated analyses (3-4 times). On the basis of statistical evaluation a dendrogram was set up, which allows highly significantly to differentiate the varieties Kolor, Modus and Tornado. The remaining 12 analysed varieties formed 4 clusters. In addition to the RAPD markers a protocol of detection of DNA polymorphism was elaborated and optimised with microsatellite (SSR) markers. For the analyses we used 2 SSR markers (1A chromosome [Xpsp2999] and 1B chromosome [Xpsp3000]), which have been discovered in wheat (T. aestivum L.). Basing on these two SSR markers the only variety Triamant was distinguished from the clusters of the other analysed varieties.
Keywords: triticale, XTriticosecale Wittmack., DNA markers, RAPD, SSR
Grants and funding:
Supported by the Czech Science Foundation, project No. 521/03/P173.
Received: March 23, 2006; Published: December 3, 2014 Show citation
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