Acta Univ. Agric. Silvic. Mendelianae Brun. 2005, 53(2), 99-106 | DOI: 10.11118/actaun200553020099

Vztah mezi mikrosatelitním polymorfismem makrofágového projevu lysozymového (mLys) genu a vybranými vlastnostmi mléka polských černobílých krav

Małgorzata Walczak-Wójciak1, Janusz Klupczyński1, Jan Miciński1, Martin Hošek2
1 Univeristy of Warmia and Mazury in Olsztyn, Department of Cattle Breeding, 10-718 Olsztyn, Oczapowskiego 5, Poland
2 Ústav chovu a šlechtění zvířat, Mendelova zemědělská a lestnická univerzita, Zemědělská 1, 613 00 Brno, Česká republika

Sledování proběhlo v letech 1998-2000 na dvou farmách v oblasti Zulawy Wislane. Do sledování bylo zahrnuto 52 dcer - prvotelek býka Parana, z nichž 21 bylo nositelkami alely 7 mLys-mic genu a 31 nositelkami alely 3 mLys-mic genu. V 30, 100, 150 a 200 dnech laktace byla sledována sérová a syrovátková bakteriolytická aktivita lysozymu, koncentace vybraných minerálních látek, technologické vlastnosti mléka, počet somatických buněk a bakterií. Zjištěné výsledky ukazují, že polymorfismu Lys-mic se nedá využít jako markeru pro mléčnou produkci krav. U lysozymového genu, hodnoceného jako faktor rezistence mastitid, nebyl pozorován vliv na počet somatických buněk a počet bakterií. Přesto v dalších sledováních, včetně dalších laktací, ve stádech ohrožených různými patogenními vlivy, by mohlo být využito polymorfismu Lys-mic genu při resistenci mastitid.

alela, lysozym, mastitis, somatické buňky, bakterie, laktace

Associations between microsatellite polymorphism within the macrophage expressed lysozyme (mLys) gene and milk Indices Properties in Polish Black-and-White Cows

The experiment was performed in the years 1998-2000 on two farms located near to Vistula Lowlands. The experimental materials were comprised of 52 daughters of bull Paran, among which 21 possessed the mLys-mic 7 allele and 31 - the mLys-mic 3 allele. The serum and whey bacteriolytic activity of the lysozyme, concentrations of selected mineral elements, technological properties of milk at successive the first lactation stages (30, 100, 150 and 200 days), somatic cell count and bacterial count were investigated in this study. The results obtained indicate that Lys-mic polymorphism has low suitability as a marker for milk production capacity in cows. The lysozyme gene, treated as a mastitis resistance factor, showed no effects on somatic cell count and bacterial count. Further investigations, conducted not only during the first lactation, but also during the next lactation in a herd threatened by a variety of pathogenic factors, would be carried to validate this Lys-mic gene polymorphism on mastitis resistance.

Keywords: alleles, lysozyme, mastitis, somatic cells, bacterial cells, lactation

Received: September 30, 2004; Published: December 28, 2014  Show citation

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Walczak-Wójciak, M., Klupczyński, J., Miciński, J., & Hošek, M. (2005). Associations between microsatellite polymorphism within the macrophage expressed lysozyme (mLys) gene and milk Indices Properties in Polish Black-and-White Cows. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 53(2), 99-106. doi: 10.11118/actaun200553020099
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