RT Journal Article
SR Electronic
A1 Matiska, Pavel
A1 Vejsadová, Hana
T1 Polyploidy induction in Phlox paniculata L. under in vitro conditions
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 58
IS 1
SP 101
OP 106
DO 10.11118/actaun201058010101
UL https://acta.mendelu.cz/artkey/acu-201001-0011.php
AB The objective of this work was to find an effective method of polyploidy induction using chemomutagens, colchicine and oryzalin, in diploid cultivar of Phlox paniculata 'Fujiyama' (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the explants (in vitro regenerated shoots) were tested; chemomutagen infiltration from nutrient media ("the infiltration method") and dipping of the explants in a chemical mutagen solution ("the dip method"). The highest values of tetraploid (5%), mixoploid (1.67%) frequency and polyploidization efficiency (1.25) were found in explants treated with 0.2% colchicine for 24 h in the dip method. Concentrations of 10 µM oryzalin and 0.2% colchicine for 14 d were the most effective for obtaining tetraploids in the infiltration method. The results will be exploited to other P. paniculata cultivars for breeding of this species.