RT Journal Article
SR Electronic
A1 Bobeková, Elena
A1 Tomšovský, Michal
A1 Horáček, Petr
T1 Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 56
IS 2
SP 281
OP 284
DO 10.11118/actaun200856020281
UL https://acta.mendelu.cz/artkey/acu-200802-0035.php
AB The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR-based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA.