PT  - JOURNAL ARTICLE
AU  - Bobeková, Elena
AU  - Tomšovský, Michal
AU  - Horáček, Petr
TI  - Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions
DP  - 2014 Nov 14
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 281--284
VI  - 56
IP  - 2
AID - 10.11118/actaun200856020281
IS  - 12118516
AB  - The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR-based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA.