PT  - JOURNAL ARTICLE
AU  - Sláma, Petr
AU  - Sládek, Zbyšek
AU  - Ryšánek, Dušan
TI  - Application of methods for detection of apoptosis and necrosis of bovine blood neutrophil granulocytes in vitro
DP  - 2014 Dec 3
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 107--116
VI  - 54
IP  - 5
AID - 10.11118/actaun200654050107
IS  - 12118516
AB  - The study was an in vitro analysis of bovine blood neutrophil apoptosis and necrosis based on the detection of their morphological and biochemical features. The experiment was carried out in six clinically healthy Holstein x Bohemian Red Pied crossbred virgin heifers aged 16 to 18 months. The fresh and in vitro cultivated blood were analysed by light and electron microscopy and flow cytometry. In fresh blood, non apoptotic or necrotic neutrophils were found by light microscopy. On the other hand, 3.34% apoptotic and 0.32% necrotic neutrophils were detected by flow cytometry. After four hours of incubation, 3.53% apoptotic and 1.37% necrotic neutrophils were found by light microscopy. In the same conditions, there were assessed 17.54% apoptotic and 0.58% necrotic neutrophils by flow cytometry. Correlation coefficient between light microscopy and flow cytometry in portion of apoptotic neutrophils was 0.625 (P