RT Journal Article
SR Electronic
A1 Vlašínová, Helena
A1 Havel, Ladislav
A1 Klemš, Marek
A1 Procházka, Stanislav
T1 Uptake and metabolism of 2,4-dichlorophenoxyacetic acid during induction of Norway spruce (Picea abies (L.) Karst.) somatic embryogenesis
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 53
IS 5
SP 169
OP 174
DO 10.11118/actaun200553050169
UL https://acta.mendelu.cz/artkey/acu-200505-0019.php
AB Uptake and metabolism of 2,4-dichlorophenoxyacetic acid (2,4-D) into zygotic embryos of Norway spruce (Picea abies (L.) Karst.) were studied on medium used for induction of somatic embryogenesis. The main task of this work was to study effect of longitudinal bisection of embryos, which was found as increasing the subsequent induction of embryogenic cultures. The maximal uptake of 2,4-D per one embryo was detected after 16 hours on medium in bisected embryos. The bisection increased 2,4-D uptake per embryo in first 16<sup>th</sup> hours, but then increased its release back to the medium. The metabolism of 2,4-D in bisected embryos was lower than in intact ones during first two days of culture.